Performed by high performance liquid chromatography (HPLC). Depending on the sample type and solubility, the separation requires different columns (RP, NP, HILIC) and solvents. Depending on the problem to be examined, we have the option of using different detectors RI, UV, FL, ELSD. If references to identify unknown substances are not available, it is possible to fractionate the sample and determine the molecular weight by MALDI mass spectrometry. In the case of substances with a low Boling point, analysis by GC-MS is a very established technique.