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Announcement:

1) The seminar will be at the Praktikums-HS and starts @4:00 pm c.t.

2) Please have a carefull look at the program. We have several special appointments this semester!


Wednesday 19.02.2020, 15:30 Uhr c.t., Seminarraum BA 02B !!!SONDERTERMIN!!!

Prof. Kira Astakhova, DTU Denmark

Novel reagents for chemical oligonucleotide synthesis: Approaching yields, stereoselectivity and biological performance

 

Abstract:

With five oligonucleotide drugs being currently approved by FDA, and over 1000 gene therapy candidates in trials, it becomes crucial to learn how we can synthesize biologically relevant backbone modified DNA and RNA in a stereoselective, cost effective and environmentally friendly way. A scientific challenge which my group at I aim at is the general approach to how we make oligonucleotides chemically today.

To address this, we carry out studies in three main directions: 1) tandem synthesis of oligonucleotides vs. conventional synthesis which is done for one strand at a time; 2) developing new solid phases for chemical oligonucleotide synthesis; 3) prepare stereoselective phosphoramidite reagents to secure the pure stereochemical outcome of backbone modified oligonucleotides. An exciting aspect of our work, that I will be presenting, is that stereochemically pure oligonucleotides have dramatically different biological properties than the currently applied mixtures of stereoisomers. Moreover, with this project we have a potential to remarkably reduce amount of waste produced during the oligonucleotide synthesis as well. Given a growing demand on high amounts of modified oligonucleotides, for diagnostics and therapy of human diseases, this is a highly appealing aspect of the project.